||Glucose-6-phosphate (6GP) is an intermediate product of glycolysis and pentose phosphate pathway, which is widely present in animals, plants and microorganisms. In the first step of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis; and in the pentose phosphate pathway, glucose-6-phosphate is its first substrate, and this process is also the main pathway for the production of NADPH. In addition, glucose-6-phosphate can also be converted into glycogen or starch and stored. Glucose 6-phosphate dehydrogenase can catalyze 6PG and NADP+ to produce 6-phosphogluconate and NADPH.NADPH makes WST-8 appear orange-yellow under the action of 1-mPMS. The absorbance is measured at 450 nm.