Mitochondria play an important role in the pathophysiology of many neurological diseases, and some neurodegenerative diseases are closely related to synaptic damage and synaptic mitochondrial dysfunction.
However, the evaluation of mitochondrial dysfunction and the efficacy of mitochondrial-targeted therapy in experimental models of neurodegenerative diseases and central nervous system injuries are limited by mitochondrial separation techniques. At present, density gradient ultracentrifugation (UC) is the only technique that can separate synaptic and non-synaptic mitochondrial subsets, but the yield of mitochondria prepared by this technique is low.
(−) technique does not have the capability or is a disadvantage. (+) technique has the capability, can perform well, or is an advantage. (++) technique has superior capability or has greater advantage compared to other methods. ( Hubbard, W.B., et al.,2019)
To solve this limitation, Creative Biogene uses the fractionated mitochondrial magnetic separation (FMMS) method to separate functional synaptic and non-synaptic mitochondria from the mouse cortex and hippocampus without the use of UC. Compared with UC, the synaptic mitochondrial protein production produced by the FMMS method is 3 times higher than that of UC.
A common problem with UC is that it is unable to obtain sufficient synaptic mitochondrial production from a low initial tissue volume for technical analysis. FMMS method can increase the production of brain-derived mitochondria be used for mitochondrial assessment, and can better detect mitochondrial damage in central nervous system injury and neurodegenerative diseases.
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