The detection of mitochondrial gene expression profile is of great significance for disease research. On the one hand, it can be applied to the screening of disease biomarkers. On the other hand, it can provide clues for the study of gene function and help to reveal the molecular mechanism of the occurrence and development of the disease.
Mitochondrial gene expression microarray means that a large number of mtDNA fragments or oligonucleotides are fixed on hard carriers such as glass, silicon, plastic, and other hard carriers to make a gene chip. After the mRNA in the sample is extracted, cDNA is obtained by reverse transcription and labeled with fluorescence in the process. After hybridization with the mtDNA chip containing thousands of genes, the fragments that do not bind on the chip are washed out, and the glass slides are scanned by laser confocal scanning. The fluorescence intensity of each point on the chip is measured, and the expression level of various genes in the sample to be tested is calculated.
Creative Biogene provides you with genome-wide expression profile chip technical services. Provide us with well-preserved tissue or cell samples, then our scientists will complete the testings and record the procedures and results in expriment reports for you. Creative Biogene chip technical service personnel can complete all the experimental operations for you, and provide a complete experimental report. And according to your research needs, Creative Biogene also provides personalized in-depth data mining services.
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