Analysis of Mitochondrial Carbon Metabolism
Creative Biogene uses ACQUITY UPLC/TripleQuad5500 combined with HPLC analysis to efficiently and accurately detect and analyze mitochondrial carbon metabolism-related substances content changes. Besides, we also provide a variety of auxiliary testing means to meet the different needs of customers around the world.
Our mitochondrial carbon metabolism analysis mainly includes substance analysis related to carbon metabolism and central carbon metabolism. Some amino acids can form a group containing one carbon atom in the process of metabolism and participate in the biosynthesis process by transfer. These groups containing one carbon atom are called a carbon group or C1 unit. The metabolism related to the formation and transfer of the C1 unit is called one-carbon metabolism. Central carbon metabolism (CCM) plays a very important role in the metabolic network, and it is an important component of the pathway of carbon from nutrients to biomass. Central carbon metabolism includes the TCA cycle, pentose phosphate pathway, and glycolysis pathway. Creative Biogene established an analytical method based on ACQUITY UPLC/TripleQuad5500 and HPLC, which can quantitatively and qualitatively analyze many kinds of substances involved in carbon metabolism.
Common metabolites or metabolites of the same kind can be tested in conjunction with standard samples. For rare carbon metabolites, Creative Biogene can provide custom testing if you can provide the standard sample. And for the enzymes in the process of carbon metabolism, Creative Biogene also has the corresponding kit to detect them using biochemical methods, to meet your different needs.
General Strategy
- Quantification of amino acids, biogenic amines, vitamins involved in one-carbon metabolism pathway by HPLC.
- Quantification of nucleotides, sugar phosphates, enzyme co-factors, TCA cycle intermediates, carboxylic acids, and phospho-carboxylic acids involved in Central Carbon Metabolism by UPLC-MRM/MS.
At the end of the experiment, Creative Biogene will provide a detailed technical report, including the SOP, relevant MS parameters, quantitative or quantitative information of identified substances, MS charts, and reliable, detailed original data.
Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.
Our Advantages
- Rich experience in the analysis of biomarker
- Outstanding research team
- Unique integrated service for mitochondrial research
- Reliable data and results
- Rapid turnaround and cost-effective
Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.
For Research Use Only. Not For Clinical Use Or Individual Users.
Online Inquiry
