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Yeast Expression System


CD Mitochondria has built a complete mitochondrial protein expression technology platform, which can provide a series of services such as plasmid construction, gene expression, protein purification, protein expression, and so on.

The yeast expression system has the advantages of prokaryotic and eukaryotic systems: simple culture conditions, fast growth, high expression level, simple operation, and low cost. The mitochondrial protein can be correctly processed, modified, and folded reasonably after translation. It can improve the solubility of the protein, and it is an ideal tool for the production and preparation of recombinant mitochondrial protein.

CD Mitochondria has rich experience in yeast protein expression and has successfully expressed more than 100 recombinant mitochondrial proteins. And we have the service of screening yeast expressing high copy strains, which can meet a variety of special needs of customers around the world. We successfully expressed disulfide bond protein and glycosylated protein by yeast expression system, which produced the protein with a similar natural structure and post-translational modification and ensured the activity of the protein. In the process of yeast system expression, the control of induction time and temperature, the grasp of culture conditions and strain growth cycle directly affect the success or failure of recombinant protein expression. CD Mitochondria's technicians have grasped and controlled the integration process to improve the success rate and expression level of expression.

Work Flow

  • Construction of Recombinant yeast expression plasmid.
  • Recombinant plasmid transformation.
  • Recombinant expression screening.
  • Expression and purification of small-scale protein (2L medium culture product).
  • Large-scale mitochondrial protein expression and purification (more than 10L)

CD Mitochondria always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

Technical characteristics

  • High productivity: unique secretory signal sequence ensures large secretion of recombinant mitochondrial protein.
  • Two kinds of expression systems can be selected: selective expression or inducible expression can be selected.
  • Mass production: bioreactor up to 500L ensures the production of gram grade levels proteins.

Multiple strains and vectors are available: CD Mitochondria provide multiple strains and carriers including X33, GS 115, SMD 1168, etc.

CD Mitochondria has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

For Research Use Only. Not For Clinical Use.

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