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Mammalian Cell Expression System


CD Mitochondria has built a complete mitochondrial protein expression technology platform, which can provide a series of services such as plasmid construction, gene expression, protein purification, protein expression, and so on.

The mammalian cell expression system can provide post-translational modification close to the natural state for recombinant mitochondrial proteins, which means protein folding and polymerization can occur close to natural proteins in the process of mitochondrial protein expression. These proteins will have the necessary spatial structure and modification of active proteins. The activity of foreign proteins produced by post-translational reprocessing modification of mammalian cells is higher than that of prokaryotic expression systems and eukaryotic expression systems such as yeast and insect cells and is closer to natural proteins.

CD Mitochondria has established a mature mammalian protein expression system and purification service platform to provide a mammalian protein expression system expression and purification services. CD Mitochondria' mammalian cell protein expression technology team has rich experience in CHO and HEK293 cell expression and can provide a variety of special requirements such as label removal, endotoxin removal, serum-free suspension culture, etc., to provide the best service to customers around the world.

Work Flow

  • Codon optimization, vector construction, and subcloning of target genes.
  • Transfection of mammalian cells to screen cells with stable transfection.
  • Evaluation of recombinant mitochondrial protein expression.
  • Purification and identification of the mitochondrial protein.
  • Large-scale mitochondrial protein expression and purification (optional).

CD Mitochondria always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

Technical characteristics

  • Expression level: our vector can highly express the recombinant mitochondrial protein in mammalian cells.
  • A variety of expression systems are available: transient expression, stable cell line expression, nutritional deficiency cell line expression (DHFR/GS), large-scale suspension cell expression, and so on.
  • Production: our serum-free suspension cell culture (HEK293 and CHO) can produce several grams of protein within a month.
  • Success rate: multiple proteins have been successfully expressed in our mammalian expression system.

CD Mitochondria has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

For Research Use Only. Not For Clinical Use.

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