When evaluating the disease model and the mitochondrial function of cancer and diabetes, intracellular metabolism is used as a common index. Lactic acid is one of the main metabolic pathways of a cells-the metabolite of glycolysis, and it is also an important biomarker of muscle fatigue and hyperelastic acidemia. It can also be used as a marker for monitoring the changes in the intracellular metabolic pathway. Besides, recent metabolic studies have shown that lactic acid is the main source of carbon in the tricarboxylic acid cycle of tissues and cancer cells.
CD Mitochondria provides quantitative detection of lactic acid produced by glycolysis metabolism and uses the optimized quantitative process.
The content of lactic acid in cell culture medium and cells can be quantified by preparing a standard curve to quantify the concentration of lactic acid in the sample and measuring the color reaction of WST.
In addition to the above methods, CD Mitochondria also provides gas chromatography for the determination of mitochondrial lactic acid content. The samples are first esterified with methyl ester, extracted with chloroform, and dried with nitrogen. The residue is dissolved in heptane before injected into the sample to determine the content of mitochondrial lactic acid by gas chromatography. CD Mitochondria always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.
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