Protein unlabeled quantification (label-free) is a mass spectrometric analysis of protein hydrolyzed peptides by LC-MS/MS technology. It does not use expensive stable isotope tags as internal standards. It works by analyzing the mass spectrometry data produced during large-scale protein identification and comparing the corresponding peptides' signal intensity in different samples to quantify the proteins corresponding to the peptides. Compared with iTRAQ technology, the sample volume required for label-free technology is small, and the cost is low. The label-free technique can be used for quantitative comparative analysis of high-throughput biomarker screening, protein-protein interaction, protein post-translational modification, and so on.
CD Mitochondria uses Thermo Fisher's Q ExactiveHF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, Orbitrap Fusion Lumos mass spectrometry platform, and Nano-LC to provide the label-free quantitative proteome analysis service. Tell us the goal of your experiment and send us your cells, and we will be responsible for all the follow-up matters of the project, including cell culture, cell labeling, protein extraction, protease digestion, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis.
CD Mitochondria will provide you with a detailed technical report when the project is delivered, including experimental steps, relevant MS parameters, details of identified amino acids, MS images (high resolution), and detailed raw data. CD Mitochondria always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.
CD Mitochondria has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.