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Determination of Mitochondrial Respiratory Chain Complex


The CD Mitochondria's detection platform uses biochemical methods to efficiently and accurately detect the activities of mitochondrial respiratory chain complex and ATP enzyme. Results can help screen mitochondrial respiratory chain complex inhibitors quickly and effectively.

Mitochondrial respiratory chain complex I (NADH oxidase) and mitochondrial respiratory chain complex II (succinate dehydrogenase) are the main elements of the electron transport chain (ETC). Complex I catalyzes the oxidation of NADH and complex II catalyzes the oxidation of succinic acid to fumaric acid. Subsequently, coenzyme Q forms coenzyme QH2, and finally leads to the decrease of terminal electron receptor O2. Mitochondrial respiratory chain complex III (cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Mitochondrial respiratory chain complex III is the gatekeeper of the mitochondrial respiratory chain and the main source of the third reactive oxygen species. Mitochondrial respiratory chain complex IV (cytochrome c oxidase) is the terminal electron receptor of the mitochondrial electron transport chain. Complex IV converts O2 to H2O through the oxidation of cytochrome c, which is related to the synthesis of mitochondrial cell membrane ATP.

Determination of Mitochondrial Respiratory Chain Complex

Mitochondrial respiratory chain complex V completes oxidative phosphorylation with the above four complexes to form ATP, which is called ATP synthetase, also known as the F0F1-ATP enzyme.

Determination of Mitochondrial Respiratory Chain Complex

The inhibition of the activity of the mitochondrial respiratory chain complex will lead to severe mitochondrial dysfunction, and long-term inhibition may lead to nervous system diseases. Therefore, the activity of the mitochondrial respiratory chain complex is often used as an indicator of neuro deficits diseases caused by mitochondrial toxicity. The CD Mitochondria detection platform uses biochemical methods to detect the activity of mitochondrial respiratory chain complex and ATP enzyme efficiently and accurately, which helps you to screen the inhibitors of mitochondrial respiratory chain complex more simply, quickly, and effectively.
General Strategy

  • The activity of complex I is calculated by measuring the oxidation rate of NADH (absorbance value at 340nm).

Determination of Mitochondrial Respiratory Chain Complex

  • The activity of complex II is reflected by measuring the decrease of DCPIP (absorbance value at 600nm).

Determination of Mitochondrial Respiratory Chain Complex

  • The activity of the complex III is reflected by measuring cytochrome C (absorbance at 550nm).

Determination of Mitochondrial Respiratory Chain Complex

  • The activity of the complex IV is reflected by measuring the oxidation rate of cytochrome C (absorbance at 550nm).

Determination of Mitochondrial Respiratory Chain Complex

  • The activity of complex V is reflected by measuring the oxidation rate of NADH (absorbance at 340nm).

CD Mitochondria always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

Related Products

CD Mitochondria seeks and provides the best product solutions for customers around the world, continuously expanding the quality of products and services, and aims to provide you with the highest quality and latest overall solutions for mitochondrial biology.

Our Advantages

  • Rich experience in the determination of mitochondrial respiratory chain complex
  • Outstanding research team
  • Unique integrated service for mitochondrial research
  • Reliable data and results
  • Rapid turnaround and cost-effective

CD Mitochondria has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

For Research Use Only. Not For Clinical Use Or Individual Users.

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