Mitochondrial protein purification is a complex process, including crude separation, preliminary purification, and fine purification. The experiment procedure includes extraction, precipitation, centrifugation, chromatography, and so on. Isolation and purification are in the downstream stage of recombinant protein expression and are closely related to the upstream process, such as whether there is an affinity tag, secretory expression, and so on.
When expressing and purifying the target protein, Creative Biogene considers the effects of upstream treatment on downstream treatment and different expression systems. Culture methods can also affect the downstream treatment process, whether the target protein expression forms inclusion bodies and target protein expression localization (intracellular, intracellular, periplasmic space and extracellular) and other influencing factors. Creative Biogene has professional mitochondrial protein purification technicians and can design a variety of protein purification experiments according to protein properties, such as affinity purification, ion exchange, molecular sieve, hydrophobic and reversed-phase, to obtain the initially purified protein, and then through the preparation of grade HPLC to obtain a pure protein with of more than 99% purity. The obtained pure protein can meet the fine experimental requirements of protein folding and recrystallization.
Creative Biogene will provide you with the experimental steps, relevant MS parameters, high-resolution MS images, raw data, and protein molecular weight, and purity analysis results immediately after the end of the experiment.Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.
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