Mitochondrial Damage Detection

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Tissue damage or cell injury can lead to mitochondrial dysfunction, which leads to oxidative damage and an increase in the production of mitochondrial ROS and the oxidation of mtDNA. Oxidized mtDNA will be released into the cytoplasm through various mechanism before released into the extracellular environment.

Figure 1. Pro-inflammatory response mediated by mitochondrial DNA. (Grazioli S., Pugin J.,2018)

The damage of mitochondria is mainly manifested in the following aspects:

• The increased permeability of the outer membrane of the mitochondria releases the substances in the membrane space between the outer membrane and the intima of the mitochondria into the cytoplasm, the most important of which is cytochrome C. the release of cytochrome C into the cytoplasm can initiate the caspase reaction, thus initiating the apoptosis received by the mitochondria.
• Decrease of mitochondrial membrane potential.
• The cardiolipin located in the intima of the mitochondria is degraded.
• 7A6 antigen exposed in mitochondria.

Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs.

General Strategy

• Detection of mitochondrial damage by gene-specific semi-quantitative PCR

Figure 2. Approach for the detection of gene-specific damage by QPCR. (Sylvette A.T., et al, 2000)

• Detection of mitochondrial damage by flow cytometry

Our Advantages

• Rich experience in mitochondrial damage detection
• Outstanding research team
• Unique integrated service for mitochondrial research
• Reliable data and results
• Rapid turnaround and cost-effective

Creative Biogene has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

References

1. Grazioli S., Pugin J. "Mitochondrial damage-associated molecular patterns: from inflammatory signaling to human diseases." Front. Immunol., 2018, 9:832.
2. Szczesny B., et al. "Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells." Sci Rep, 2018, 8:914.
3. Sylvette A.T., et al. "Analysis of gene-specific DNA damage and repair using quantitative polymerase chain reaction." Methods, 2000, 22(2): 135-147.

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