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Identification of Unknown Metabolites


Based on the demand of global researchers for the study of mitochondrial functional metabolism and regulation, CD Mitochondria, To study mitochondrial functional metabolism and regulation, CD Mitochondria combines NMR, LC-MS/MS, and bioinformatics analysis to identify unknown metabolites. We can accurately identify metabolites in complex biological samples and carry out association analysis to construct a complete network of mitochondrial metabolism and regulation mechanisms.

Unknown metabolites refer to small molecules that can be detected repeatedly but whose chemical identity has not yet been determined. Although these compounds have not been identified and classified, they can still be distinguished and quantified according to spectral data in metabolomics experiments. Mitochondria play an extremely important role as the functional hub of energy production, calcium balance, cell signal transduction and apoptosis, and intracellular substance metabolism in eukaryotic cells. Successfully identifying unknown metabolites will promote the identification of disease targets, the discovery of biomarkers, and the study of mitochondrial metabonomics.

Identification of Unknown Metabolites

CD Mitochondria combines NMR, LC-MS/MS, and bioinformatics analysis to provide mass spectrometry-based analysis services for the identification of unknown metabolites, which can accurately identify metabolites in complex biological samples. CD Mitochondria continues to develop tools and databases to automate the accurate and complete identification of all metabolites.

General Strategy

The typical LC / MS metabonomics workflow includes sample preparation steps (quenching, cell lysis, extraction, etc.), LC / MS analysis, feature discovery, and statistical analysis, with the structure confirmation of unknown metabolites as the conclusion. In the experiment and high-order analysis phase of the metabolite map, the feature annotation (identified compounds, adducts, isotopes, neutral losses, etc.), will be used in several experiments and the common background ions can be revealed and the priority of new features can be determined for further characterization.

Identification of Unknown Metabolites

CD Mitochondria always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs. For more information, you can click on Mitochondrial Metabonomics.

Our Advantages

  • Rich experience in identification of unknown metabolites
  • Outstanding research team
  • Unique integrated service for mitochondrial research
  • Reliable data and results
  • Rapid turnaround and cost-effective

CD Mitochondria has the most professional team to serve customers around the world. If you have any questions about the content of this service, please feel free to contact us. We look forward to your contact.

Reference

  1. Benjamin P. B., Trent R. N. " Dealing with the Unknown: Metabolomics and Metabolite Atlases. " Journal of the American Society for Mass Spectrometry, 2010, 21(9): 1471-1476.
For Research Use Only. Not For Clinical Use.

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