Unknown metabolites refer to small molecules that can be detected repeatedly but whose chemical identity has not yet been determined. Although these compounds have not been identified and classified, they can still be distinguished and quantified according to spectral data in metabolomics experiments. Mitochondria play an extremely important role as the functional hub of energy production, calcium balance, cell signal transduction and apoptosis, and intracellular substance metabolism in eukaryotic cells. Successfully identifying unknown metabolites will promote the identification of disease targets, the discovery of biomarkers, and the study of mitochondrial metabonomics.
Creative Biogene combines NMR, LC-MS/MS, and bioinformatics analysis to provide mass spectrometry-based analysis services for the identification of unknown metabolites, which can accurately identify metabolites in complex biological samples. Creative Biogene continues to develop tools and databases to automate the accurate and complete identification of all metabolites.
The typical LC / MS metabonomics workflow includes sample preparation steps (quenching, cell lysis, extraction, etc.), LC / MS analysis, feature discovery, and statistical analysis, with the structure confirmation of unknown metabolites as the conclusion. In the experiment and high-order analysis phase of the metabolite map, the feature annotation (identified compounds, adducts, isotopes, neutral losses, etc.), will be used in several experiments and the common background ions can be revealed and the priority of new features can be determined for further characterization.
Creative Biogene always provides you with customized services for mitochondrial research, and our one-stop research and analysis platform can meet all your research needs. For more information, you can click on Mitochondrial Metabonomics.
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