Restriction fragment length polymorphism (RFLP) is one of the most important analytical methods in molecular biology. The principle of this technique is to detect the size of specific DNA fragments formed by restriction endonuclease digestion of DNA. Therefore, all mutations in restriction sites, such as point mutations (new generation and removal of restriction sites) and recombination of DNA fragments (such as insertions and deletions leading to changes in the length between restriction sites) can lead to the production of RFLP. The RFLP probe is a labeled DNA sequence, which is hybridized with one or more fragments of digested DNA samples after separation by gel electrophoresis, showing unique imprinting patterns specific to a particular genotype at specific sites.
Terminal restriction fragment length polymorphism (T-RFLP) is an extension of RFLP, which is a microbial population mapping method based on the fluorescence-labeled primer PCR to analyze the composition of microbial population according to the length of the terminal restriction fragment. Its basic principle involves the restriction endonuclease digestion of terminal fluorescence-labeled PCR products. T-RFLP has the advantages of high speed, high repeatability, sensitivity, and high throughput.
After the end of the experiment, our customers can get the original datasheet original picture, primer sequence, professional analysis results, and complete experimental report as soon as possible, and there will be a special person to do after-sales service in the follow-up. If you have any questions about the experimental results, you can contact our staff at any time.
Creative Biogene also provides other professional technical services for polymorphism research to customers around the world, including, but not limited to, AFLP, RAPD, MSAP, SSR & ISSR, SSCP, SNP technology. If you are interested in or have questions about any of these projects, you can contact us. Our staff will provide you with 24-hour considerate service.