||mtCheck™ Phosphoenolpyruvate Carboxylase Assay Kit (Microplate) (KITA1032)
||Phosphoenolpyruvate carboxylase (EC 22.214.171.124) is widely present in animals, plants, microorganisms and cells. It is an enzyme that catalyzes the reaction of phosphoenolpyruvate with carbon dioxide to produce oxaloacetate and is an irreversible reaction. It plays an important role in regulating the operation of the tricarboxylic acid cycle. Phosphoenolpyruvate carboxylase catalyzes phosphoenolpyruvate and CO2 to produce oxaloacetate and HPO42-, and malate dehydrogenase further catalyzes oxaloacetate and NADH to produce malate and NAD+. The NADH reduction rate is measured at 340nm and the PEPC activity is calculated.
||Store components of the kit at 4°C or -36°C
||Our products are intended for research use only and are not to be used for other purposes, including but are not limited to in vitro diagnostic uses, ex vivo or in vivo therapeutic uses.
||Absorbance microplate reader