||Phosphofructokinase (EC 18.104.22.168) is widely present in animals, plants, microorganisms and cultured cells. It is responsible for the conversion of fructose-6-phosphate and ATP into fructose-1,6 diphosphate and ADP, and is a key regulatory enzyme in the glycolysis process. one. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1,6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The decrease rate of NADH can be measured at 340nm to reflect the activity of Phosphofructokinase.